CAT (chloramphenicol acetyl transferase) activity of several different promoters transfected into rat XC cells. The variant MMTV promoter examined here functions in virtually any cell line that contains a functional glucocorticoid receptor (GR). The promoters included in this figure are:

  1. SV40 promoter and enhancer
  2. MMTV wild-type promoter with dexamethasone
  3. MMTV wild-type promoter without dexamethasone
  4. MMTV variant #1 promoter with dexamethasone
  5. MMTV variant #1 promoter without dexamethasone
  6. MMTV variant #4 promoter with dexamethasone
  7. MMTV variant #4 promoter without dexamethasone
  8. MMTV variant #2 promoter with dexamethasone
  9. MMTV variant #2 promoter without dexamethasone
  10. pCAT Basic
  11. no enzyme, cell lysate
  12. LacSwitchTM, fully induced (with IPTG)
  13. LacSwitchTM, with lac repressor plasmid co-transfected

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