CAT (chloramphenicol acetyl transferase) activity of several different promoters
transfected into rat XC cells. The variant MMTV promoter examined here functions
in virtually any cell line that contains a functional glucocorticoid receptor
(GR). The promoters included in this figure are:
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SV40 promoter and enhancer
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MMTV wild-type promoter with dexamethasone
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MMTV wild-type promoter without dexamethasone
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MMTV variant #1 promoter with dexamethasone
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MMTV variant #1 promoter without dexamethasone
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MMTV variant #4 promoter with dexamethasone
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MMTV variant #4 promoter without dexamethasone
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MMTV variant #2 promoter with dexamethasone
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MMTV variant #2 promoter without dexamethasone
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pCAT Basic
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no enzyme, cell lysate
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LacSwitchTM, fully induced (with IPTG)
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LacSwitchTM, with lac repressor plasmid co-transfected
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